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DNA Purification Kits

EXTRACTME DNA CLEAN-UP & GEL-OUT KIT - NEW VERSION (EM26.1)

Catalog No. size price Add to basket
EM26.1-010 10 extractions

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EM26.1-050 50 extractions

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EM26.1-250 250 extractions

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The EXTRACTME DNA CLEAN-UP & GEL-OUT KIT is designed for a rapid and efficient purification of DNA fragments after enzymatic reactions and directly from agarose gels (standard and low-melting point agarose gels run in either a TAE or TBE buffer). It efficiently removes nucleases, enzyme inhibitors, detergents, restriction enzymes, polymerases, divalent ions, agarose, ethidium bromide and other contaminants. The purified DNA can be used in common downstream applications. The kit enables the purification of DNA fragments from 50 bp to 20 kb, as well as plasmid and genomic DNA. However purification of fragments smaller than 100 bp and larger than 10 kb will result in decreased recovery rates. The purification protocol and buffer formulations were optimized for high yields and purity of DNA. The product is intended for research use only.

 

 

SAMPLE MATERIAL

 

CLEAN-UP: up to 200 μl of a DNA sample

GEL-OUT: agarose fragment of up to 300 mg containing DNA

 

YIELD

 

Depending on DNA fragment length (in the range of 100 bp – 10 kb):

CLEAN-UP: 60-99%

GEL-OUT: 70–95%

 

 

DNA FRAGMENT LENGTH

 

100 bp – 10 kb
DNA fragments in the range of 50–100 bp and 10–20 kb can also be purified
as can genomic and plasmid DNA, however the efficiency will be decreased.

 

 

BINDING CAPACITY

 

Approx. 40 μg DNA

 

TIME REQUIRED

 

5–10 min for clean-up procedure

16–20 min for gel-out procedure

 

 

DNA PURITY

 

A260/A280 = 1,7-1,9

 

QUALITY CONTROL

 

The quality of each production batch (LOT) of the EXTRACTME DNA CLEAN-UP & GEL-OUT KIT is tested using standard QC procedures. Purified DNA concentration and quality are evaluated by gel electrophoresis and spectrophotometer.


Manufacturer: DNA-GDAŃSK

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